Journal: International Journal of Pharmaceutics: X

Article Title: Comparing the transfection efficiency of cationic monomer ratios in vinylimidazole and aminoethyl methacrylate copolymers

doi: 10.1016/j.ijpx.2025.100327

Figure Lengend Snippet: (A) Cell viability of HeLa cells after 48 h of incubation with optimum polyplex of each copolymer (containing 1 μg plasmid DNA). * shows significant difference ( p < 0.05) with AV/pDNA group. (B) Percentage of transfected HeLa cells, determined by measurement of GFP expression using flow cytometry. X shows p < 0.05 compared to DNAfectamin group, # shows p < 0.05 compared to AV/pDNA group and * symbol shows significant difference (p < 0.05) between the compared groups on the fig. (C, D) Flow cytometry histograms and scatter plots of transfected cells by optimum polyplex of each copolymer, respectively.

Article Snippet: 2-Aminoethyl methacrylate hydrochloride (90 %, stabilized) was from Acros Organics (New jersey, USA), 1-vinylimidazole, 2,2 azobisisobutyronitrile (AIBN), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma–Aldrich (St. Louis, MO, USA), dimethyl sulfoxide (DMSO) was from Merck Chemical Company (Darmstadt, Germany), fetal bovine serum (FBS), trypsin and Dulbecco's modified Eagle's medium (DMEM) were purchased from BioIDEA (Tehran, Iran), L929 and HeLa cell lines were obtained from the Pasteur Institute (Tehran, Iran), DNA size marker (100–10,000 bp), 6× DNA loading dye, DNA Safe Stain and Agarose gel were supplied from SinaClon BioScience (Iran), pLOX-CWgfp plasmid was from Addgene (USA) and DNA-fectamine was purchased from Bio Basic (Toronto, Canada).

Techniques: Incubation, Plasmid Preparation, Transfection, Expressing, Flow Cytometry